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adblockerx of somatostatin from rat brain. Somatostatin is a tetradecapeptide, found in high concentrations in the central nervous system, which exhibits powerful inhibitory effects on the secretion of several hormones, mainly growth hormone and insulin. Here we report the purification of a somatostatin-like molecule from rat brain. The crude extract was first purified on a diethylaminoethyl-cellulose column and further on a Sephadex G-25 column. The fraction with somatostatin activity was eluted from a Sephadex G-25 column by stepwise elution with 0.3 M NaCl, yielding a single pure and active fraction. The crude extract was further purified by reverse-phase high-performance liquid chromatography and high-performance liquid chromatography-reverse-phase high-performance liquid chromatography. On the basis of circular dichroism, mass spectroscopy and amino acid analysis, this pure, partially purified material appeared to be a somatostatin-like molecule. The overall yield of this method was 13%.Your risk of developing of eating disorders is increased if you are unhappy about your appearance and body. Whether you experience eating disorders as a result of gaining weight or losing weight, dieting will increase your risk. Helping someone who has an eating disorder is very difficult. We don’t know what caused the person to develop the disorder, so we can’t offer advice on what to do. What are the signs of an eating disorder? The person may start avoiding food or eating and then, start bingeing on food. These behaviours can lead to weight loss and in some cases, the person will lose weight despite eating. The person’s weight loss can be difficult to stop once they begin and even when they lose weight they may want to gain it back. In these cases, the person may eat whatever they want, whenever they want, even if they are not hungry. Some people who have



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